Part:BBa_K5252021:Design

tetR repressor

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimized using Genscript for E. coli, while removing SapI, BsaI, BbsI, PaqCI, Esp3I, & FaqI.

Source

tetR inhibitor for the pTet promoter inducible by aTc: https://static-content.springer.com/esm/art%3A10.1038%2Fnature11516/MediaObjects/41586_2012_BFnature11516_MOESM110_ESM.pdf